Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line

Mohd Hussain, Nurlaili Najmie and Uzir, Bisha Fathamah and Mohd Hassan, Norazian (2016) Arborinine from Glycosmis Pentaphylla (retz.) DC induces apoptosis through activation of Caspase-3/7 in human mammary gland Adenocarcinoma (MCF-7) cell line. In: 2nd ICIP (International Conference on Industrial Pharmacy) 2016, 15-16th August 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Arborinine is an acridone alkaloid found exclusively in Rutacae family has been shown to inhibit proliferation of a wide variety of cancer cell lines. The present study is aimed to isolate and investigate the anti-proliferative activity of arborinine, isolated from a Malaysian Rutaceae, G. pentapylla (Retz. D) on human mammary gland adenocarcinoma, MCF-7 cell line. Isolation and characterization of arborinine were conducted from the leaves of G.pentaphylla collected from Kelantan via acid-base extraction and wet column chromatographic techniques. The structure of arborinine was elucidated by comparing its spectroscopic data with published reports. Arborinine was studied for its anti-proliferative activity with the help of MTT assay and further with TUNEL and caspase-3/7 assay to investigate the apoptosis mechanism. The result showed that 0.1295g (12.15%) of arborinine was yield from 1.0653g of alkaloidal extract. Arborinine inhibited the proliferation of MCF-7 in a dose- and time-dependent manner with IC50 (6.25± 1.38µM). Dark brown apoptotic nuclei were observed as a result of DNA fragmentation via TUNEL assay further confirmed the mode of cell death via apoptosis. Activation of caspase-3/7 may suggest the apoptosis mechanism induced through the mitochondrial pathway on MCF-7 cells. The data presented suggest that arborinine can be a potential structure for further investigation in the development of a new anticancer agent.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5634/54885
Uncontrolled Keywords:	Arborinine, Glycosmis Pentaphylla (retz.) DC,Adenocarcinoma (MCF-7) cell line, Caspase-3/7
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Asst. Prof Bisha Uzir
Date Deposited:	21 Feb 2017 10:01
Last Modified:	21 Feb 2017 10:01
URI:	http://irep.iium.edu.my/id/eprint/54885

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