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In vitro and in vivo gene expression studies of cartilage-like tissue engineered construct using a combination of transiently transfected human osteoarthritic chondrocytes and tissue engineering technique

Sha'ban, Munirah and Mohamad Sukri, Norhamiza and Zulkifly, Ahmad Hafiz (2016) In vitro and in vivo gene expression studies of cartilage-like tissue engineered construct using a combination of transiently transfected human osteoarthritic chondrocytes and tissue engineering technique. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 21-25 Nov 2016, Pulau Pinang.

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Abstract

Purpose: To evaluate the formation of cartilage-like tissue engineered constructs (TECs) using human osteoarthritic chondrocytes overexpressed with SOX9 gene seeded on poly(lactic-co-glycolic acid) (PLGA) with and without fibrin scaffolds through cartilaginous genes expression analysis. Methods: Upon approval by the IREC18; NMRR-12-1383-14531 and IIUM/IACUCApproval/2015/[5][22], six cartilage samples were obtained from consented patients after joint replacement surgery. The cells were isolated, cultured and transfected with pcDNA3-SOX9 using Lipofectamine 2000™. Prefabricated disc-shaped porous PLGA with and without fibrin were used as scaffolds. The ‘cells-scaffolds’ TECs were formed and cultured for 3-week and implanted subcutaneously at the dorsum of athymic mice for 4-week. Collagens I, II, IX, X, XI, SOX9 and aggrecan expression were evaluated using a qualitative two-step reverse-transcriptase PCR. GAPDH and β-actin genes were used as internal controls. Results: Presence of cartilaginous markers can be detected in all TECs with various expression intensity. Collagen II, the cartilage-specific marker was down-regulated in vitro but re-expressed in vivo. Collagen I, X, SOX9 and aggrecan were steadily expressed in all TECs. Although collagen IX and XI are closely associated with collagen II, their expressions were almost untraceable except for few cases. Presence of GAPDH and β-actin genes indicated the reliability of the analysis. Conclusion: Cartilage-like TECs have been successfully formed based on genes expression analysis.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 6006/54740
Uncontrolled Keywords: Cartilage, Tissue Engineering, in vivo, in vitro, gene transfer, PLGA, SOX9
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Munirah Sha'ban
Date Deposited: 28 Jan 2017 01:31
Last Modified: 21 Mar 2017 10:28
URI: http://irep.iium.edu.my/id/eprint/54740

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