Alias, Norsyuhada and Leow, Adam Thean Chor and Mohamad Ali, Mohd. Shukuri and Salleh, Abu Bakar and Ahmad Tajudin, Asilah and Raja Abd. Rahman, Raja Noor Zaliha
(2016)
Antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract.
In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-6th August 2016, Kuantan, Pahang.
Abstract
Antilipase from natural resources are a potential tool for the treatment of obesity while antioxidant-rich plants are essential in combating degenerative diseases. The aim of this study is to determine the antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract. The inhibitory activity against pancreatic lipase was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Antioxidant activity of O. stamineus extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The O. stamineus crude extract exhibited strong lipase inhibitory activity with an IC50 value of 34.7 µg/ml. The inhibition mode study disclosed that O. stamineus could act as uncompetitive inhibitor. O. stamineus showed high antioxidant activity with an EC50 value of 26.3 µg/ml. The results suggest that O. stamineus has shown potential as a source of natural antilipase and antioxidant.
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