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Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas)

Sheikh Mohamed, Hassan Ibrahim and John, Akbar and Yunus, Kamaruzzaman and S., Solahuddin (2016) Effect of captive breeding on the Amoebocyte cell viability in Malaysian horseshoe crab (Tachypleus gigas). In: The 14th Symposium of Malaysian Society of Applied Biology (MSAB 2016), 29th–31st May 2016, Melaka.

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Amoebocyte cells degranulate in response to the bacterial pyrogen, which is the basis for the detection of endotoxin in various medical applications. It is presumed that the captive breeding might impart the cellular reaction by altering their functionality. Crabs under 4 month captivity and wild crabs were bled in triplicate to determine cell viability and density. A total of 3 anticoagulants formulations (A, B and C) were used to obtain the amoebocyte cells. Results showed that the significant reduction in the cell viability of captive crabs compared to the wild crabs (P<0.01). The effect of different anticoagulants on cell viability was also apparent in this study. Cell viability of captive crabs in anticoagulant A, B and C were 40%, 58% and 52% respectively. Whilst, the cell viability of wild crabs was 80%, 86% and 84% respectively. On the contrary, mean cell density in captive crabs were higher compared to the wild crabs (P<0.05) with the mean value of 8×106 cells/ml and 6×106 cells/ml in captive and wild crabs respectively. The performance of different anticoagulants used in this study was in the order of B>C>A (refer methodology). We conclude that the captive breeding has profound negative effect on the amoebocyte viability.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 7130/54269
Uncontrolled Keywords: Tachypleus gigas; anticoagulant; living fossil; amoebocyte cells; captive breeding.
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Kulliyyah of Science > Department of Marine Science
Depositing User: Dr. Akbar John
Date Deposited: 03 Feb 2017 10:40
Last Modified: 03 Feb 2017 10:40
URI: http://irep.iium.edu.my/id/eprint/54269

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