Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana

Aksoy, Hasan Murad and Kaya, Yilmaz and Tengku Abdul Hamid, Tengku Haziyamin (2017) Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana. Biotechnology and Biotechnological Equipment, 31 (1). pp. 85-90. ISSN 1310-2818 E-ISSN 1314-3530

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Abstract

In the Erwinia amylovora genome, the hrp gene cluster containing the dspA/E/EB/F operon plays a crucial role in mediating the pathogenicity and the hypersensitive response (HR) in the host plant. The role of the dspA/E gene derived from E. amylovora was investigated by monitoring the expression of the b-glucuronidase (GUS) reporter system in transgenic Arabidopsis thaliana cv. Pri-Gus seedlings. A mutant DdspA/E strain of E. amylovora was generated to contain a deletion of the dspA/E gene for the purpose of this study. Two-week-old seedlings of GUS transgenic Arabidopsis were vacuum-infiltrated with the wild-type and the mutant (DdspA/E) E. amylovora strains. The Arabidopsis seedlings were fixed and stained for GUS activity after 3–5 days following infiltration. The appearance of dense spots with blue staining on the Arabidopsis leaves indicated the typical characteristic of GUS activity. This observation indicated that the wild-type E. amylovora strain had induced a successful and efficient infection on the A. thaliana Pri-Gus leaves. In contrast, there was no visible GUS expression on leaf tissues which were inoculated with the DdspA/E mutant E. amylovora strain. These results indicate that the dspA/E gene is required by the bacterial cells to induce HR in non-host plants.

Item Type:	Article (Journal)
Additional Information:	4261/53950
Uncontrolled Keywords:	GUS activity; Erwinia amylovora; Arabidopsis thaliana; dspA/E gene; HR
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	10 Jan 2017 12:13
Last Modified:	25 Apr 2018 13:53
URI:	http://irep.iium.edu.my/id/eprint/53950

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