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Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243

Arzmi, Mohd Hafiz and Abdul Razak, Fathilah and Musa, Md Yusoff and Wan Harun, Wan Himratul Aznita (2012) Effect of phenotypic switching on the biological properties and susceptibility to chlorhexidine in Candida krusei ATCC 14243. FEMS Yeast Research, 12 (3). pp. 351-358. ISSN 1567-1356 E-ISSN 1567-1364

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Abstract

Phenotypic switching is characterized as a virulence factor of Candida spp. This study was carried out to evaluate the phenotypic switching ability of C. krusei ATCC 14243 and to determine its effect on the biological properties, adherence capacity and susceptibility towards chlorhexidine digluconate (CHX). To induce switched generations C. krusei was cultured under nitrogen-depleted growth conditions by adding phloxine B. These phenotypically switched colo- nies were designated as the 1st generation. Subsequent sub-culturing was per- formed to produce the 2nd, 3rd and 4th switched generations. The recovery of the 3rd generation was the highest at 85.7% while that of the 4th generation was lower at 70.8%, and the recovery of the 1st and 2nd generations gradually reduced to 46.6% and 36.4%, respectively. All generations of C. krusei were susceptible towards CHX. The unswitched C. krusei was the most susceptible but the least adherent to coated hard surfaces. The 2nd generation was the least susceptible, but with the highest adherent ability. The minimum inhibition concentration and minimal fungicidal concentration of C. krusei of all genera- tions were determined at 0.4 mg mL 1. These observations suggest that the switching activity of C. krusei induces changes to its biological properties and susceptibility towards CHX.

Item Type: Article (Journal)
Uncontrolled Keywords: susceptibility; adherence; saliva-coated surfaces.
Subjects: Q Science > QR Microbiology
R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): UNSPECIFIED
Depositing User: Dr Mohd Hafiz Arzmi
Date Deposited: 30 Dec 2016 11:42
Last Modified: 30 Dec 2016 11:57
URI: http://irep.iium.edu.my/id/eprint/53621

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