Effect of different drying treatments and solvent ratios on phytochemical constituents of ipomoea aquatica and correlation with α-glucosidase inhibitory activity

Abu Bakar Sajak, Azliana and Abas, Faridah and Ismail, Amin and Khatib, Alfi (2016) Effect of different drying treatments and solvent ratios on phytochemical constituents of ipomoea aquatica and correlation with α-glucosidase inhibitory activity. International Journal of Food Properties, 19 (12). pp. 2817-2831. ISSN 1094-2912 E-ISSN 1532-2386

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Abstract

Ipomoea aquatica is an aquatic plant that is widely consumed in Southeast Asia as a vegetable. In this study, the influence of various ethanol ratios (0, 20, 50, 80, and 100%) as an extraction solvent and different drying methods including air drying (AD), sun drying (SD) and oven drying (OD) on phytochemical constituents of I. aquatica was investigated using a proton nuclear magnetic resonance (1H NMR) based metabolomics approach. The effect on αglucosidase inhibitory activity and total phenolic content (TPC) was also examined. Clear discrimination was observed between different ethanol ratios and different drying processes by principal component analysis (PCA). The highest α-glucosidase inhibitory activity was observed for absolute ethanol extract from the OD method with IC50 value of 204.0 ± 59.0 µg/mL and TPC value of 22.0 ± 0.7 µg GAE/mg extract. Correlation between the α-glucosidase inhibitory activity and the metabolite were analyzed using a partial least square (PLS) analysis. The metabolites that are responsible for the activity were quercetin derivatives, chlorogenic acid derivatives, sucrose and fructose. This study highlights the basis for future investigations of I. aquatica as a source of food that has the potential for nutraceutical enhancement and as ingredient in medicinal preparation.

Item Type:	Article (Journal)
Additional Information:	6915/53531
Uncontrolled Keywords:	1H NMR metabolomics, Ipomoea aquatica, Alpha-glucosidase inhibitory, PLS
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	13 Dec 2016 16:53
Last Modified:	04 Oct 2017 08:26
URI:	http://irep.iium.edu.my/id/eprint/53531

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