Assessment of oxidative stress status and neuroprotection by vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats

Saxena, Anil Kumar and A.Talib, Norlelawati and Azzubaidi, Marwan Saad and Sayeed, Sayyada (2015) Assessment of oxidative stress status and neuroprotection by vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats. Project Report. IIUM, Selangor. (Unpublished)

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Abstract

Background: A persistent reduction in regional cerebral blood flow (CBF) compromises memory and cognitive functions in the elderly leading to neurological illnesses. To unravel the neuropathological consequences of a reduced CBF, a similar condition has been created in rats by common carotid artery occlusion (2 vessel occlusion, 2VO). Oxidative stress, caused due to a disproportionate release of reactive oxygen species (ROS), has been associated with neuronal cell death in various regions of the brain. Objective: To study the antioxidant and neuroprotective effects of Vitamin E in chronic cerebral hypoperfusion induced neurodegeneration in rats. Methods: After acclimatization, twenty four Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B–2VO, and Group C–2VO+E (treated daily with Vit E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. The Isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels Results: There was significant difference in neuronal cell death in 2VO group as compared to sham group. In 2VO+E rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher (p<0.05) as compared to the 2VO group. Moreover, Iso-F2 levels in 2VO group was significantly higher (p<0.05) as compared to 2VO+E group, implying high oxidative stress in 2VO group and reduction of oxidative stress levels in 2VO+E group. Conclusion: This study clearly demonstrated the effectiveness of Vit E as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion induced-neurodegeneration in rats.

Item Type:	Monograph (Project Report)
Additional Information:	5517/53292
Uncontrolled Keywords:	Neurological illness, 2VO rats, Vitamin E, Antioxidant, neurodegeneration
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine > Department of Basic Medical Kulliyyah of Medicine
Depositing User:	Dr. Anil Kumar Saxena
Date Deposited:	01 Sep 2020 13:33
Last Modified:	01 Sep 2020 13:33
URI:	http://irep.iium.edu.my/id/eprint/53292

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