Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth

Mokhtar@Makhtar, Khairani Idah and Nordin, Nur Sazwi and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Kannan, Thirumulu Ponnuraj (2016) Effects of flaxseed extract on the viability of stem cells from human exfoliated deciduous teeth. In: 6th MTERMS (Malaysian Tissue Engineering and Regenerative Medicine Scientific Meeting) 2016 in conjunction with 2nd Malaysian Stem Cell Meeting, 17th-18th Nov. 2016, Seberang Jaya, Pulau Pinang. (Unpublished)

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Abstract

Purpose: To determine the effects different concentrations of flaxseed crude extract on the viability of stem cells from human exfoliated deciduous teeth (SHED). Methods: Whole flaxseeds were ground and extracted using 99.8% ethanol in a Soxhlet chamber. The crude extract was kept in a closed container and stored at 4°C until further use. SHED (ALLCells, USA) was cultured and maintained in Alpha-MEM supplemented with FBS and antibiotics at 37°C in a CO2 incubator. Cells were trypsinised and 1000 cells were seeded in a 96-well plate. After 24 hours of incubation, SHED were treated with different concentrations of Flaxseed crude extract (0.0-16 mg/ml). The cell viability was determined using MTT assay after 3 days’ incubation. The morphology of the cells was also observed. Results: The SHED viability was more than 100% until a concentration of 8 mg/ml of flaxseed extract and an IC50 and IC25 values at 11.85 mg/ml and 10.82 mg/ml respectively which lead to cell death. Additionally, there were also morphological changes when treated with these different concentrations. Cells appeared healthy and maintained the fibroblast-like shape at 4 mg/ml. However, at 8 mg/ml, some of the cells cytoplasm started to demonstrate vacuolation and granulation and at 16 mg/ml, all cells appeared non-viable. Conclusions: The cell viability of SHED was more than 100% until 8 mg/ml of the flaxseed extract consistent with the morphological changes indicating the potential of this extract to be used further in research relating to tissue engineering involving stem cells.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7172/53257
Uncontrolled Keywords:	flaxseed, stem cells, human exfoliated deciduous teeth
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry
Depositing User:	Assoc Prof Khairani Idah Mokhtar
Date Deposited:	09 Dec 2016 10:49
Last Modified:	07 Mar 2017 11:29
URI:	http://irep.iium.edu.my/id/eprint/53257

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