Zainal Abidin, Zaima Azira and Ahmad, Asmat and Latip, Jalifah and Usup, Gires
(2016)
Marine streptomyces SP. UKMCC_PT15 producing undecylprodigiosin with algicidal activity.
Jurnal Teknologi, 78 (11(2)).
pp. 55-60.
E-ISSN 2180-3722
Abstract
Marine actinomycetes are now in demand as they are capable of producing unique
and novel compounds with wide biological activities. Marine Streptomyces sp.
UKMCC_PT15 previously isolated from seawater collected in Pulau Tinggi, Johor was used
in this study. Molecular identification showed high similarity of Streptomyces sp.
UKMCC_PT15 with Streptomyces fradiae and S. diastaticus subsp. ardesiacus based on
partial sequence of 16S rRNA gene. Further characterisations of this bacterium include
spore morphology using SEM, growth on various media, salt tolerance test and carbon
utilisation profile. This bacterium had straight spore chain with smooth-surfaced spores
and was able to tolerate up to 11% NaCl with capabilities of utilising >40 carbon sources.
Undecylprodigiosin (C25H35N3O) was successfully purified through succession of column
chromatography and finally using HPLC. Structure elucidation was confirmed through
NMR spectroscopy, MS and comparison with established data. This compound
demonstrated strong antibacterial activities against S. aureus, B. subtilis and C. albicans
but weak antibacterial activities against E. coli, P. aeruginosa and Methicillin-resistant S.
aureus (MRSA), Interestingly, undecylprodigiosin also demonstrated algicidal activity
when tested against toxic dinoflagellates, A. minutum and P. bahamense, both of which
are responsible for harmful algal blooms (HABs). Undecylprodiogiosin with concentration
of 10-100 μg/ml gave ~100% algicidal activity against both dinoflagellates. Further testing
with undecylprodigiosin concentration < 10 µg/ml showed that undecylprodigision was
capable of killing significantly high numbers of both dinoflagellates, giving a high
algicidal activity. Findings from this study suggested the potential use of
undecylprodigiosin as algicidal agent which could be used for the mitigation of HABs.
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