Pneumococcal hyaluronidase: a product of enzyme in normal human flora

Ahmad Yusof @ Hanafi, Hanani and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Osman, Malina and Jamal, Farida (2016) Pneumococcal hyaluronidase: a product of enzyme in normal human flora. In: 2nd World Congress on Integration and Islamicisation: Focus on Medical and Healthcare Science (2WCII 2016), 21st-23rd October 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Hyaluronidase degrades hyaluronan or hyaluronic acid (HA), a major component of extracellular matrix in human connective tissues. Among three types of hyaluronidase family members, hyaluroglucosaminidase which is extracted from vertebrate testes has been widely used in various medical or cosmetically-related procedures such as ophthalmic surgery and dermatosurgery. Animal-based proteins are often associated with the issue of halal status due either to their source or the production process. Bacteria can be an alternative source of enzyme and protein as no halal issues may be involved as in the case of animal products. Bacterial hyaluronidase has been reported to be produced by various species of microorganisms, including Streptococcus pneumoniae. Most previous studies reported pathogenic pneumococci as highly active producers of hyaluronidase, while its production by normal flora pneumococci has yet to be intensively explored. The present study attempted to compare hyaluronidase activity between normal flora and pathogenic pneumococci. A total of fifteen (n=15) pathogenic pneumococci isolated from blood of invasive pneumococcal disease patient and eleven (n=11) normal flora pneumococci isolated from anterior nares of healthy individuals were screened for hyaluronidase activity using HA agar plate and HA turbidity reduction assay. All pneumococci strains produced hyaluronidase, with higher enzymatic activity in normal flora pneumococci (p = 0.024). This finding suggests that normal flora could be a selected and studied for large scale production of hyaluronidase which more likely to be a syariah compliant and halal source of the enzyme.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7232/52880
Uncontrolled Keywords:	Pneumococcal hyaluronidase, enzyme, human flora
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	13 Feb 2017 10:04
Last Modified:	21 Feb 2017 15:27
URI:	http://irep.iium.edu.my/id/eprint/52880

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