Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis

Hamdan, Nurul Ashima and Mohammad Noor, Normawaty and Abd Hamid, Shafida and Md Zin, Noor Hasniza and Muhamad Bunnori, Noraslinda (2016) Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis. In: Medical Research Symposium and Pacific Partnership in Conjunction with Kuantan Research Day 2016, 4th August 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Protein profiling of harmful algae is an ongoing study where the latest analysis was conducted on A. minutum in 2015. It is a fundamental study where the protein expression of targeted species can be used to understand the biochemical pathway of selected proteins. Alexandrium spp. in Malaysia has the potential to cause massive blooming that brings harm to the aquatic ecosystem. Methods: In this experiment three methods of cell lysis were tested against A. leei isolated from Malaysian waters. Axenic culture of the sample was established in enriched seawater media (ESDK) with 12 hours of light and 12 hours of dark conditions. The sample was extracted during exponential phase (day 18) where the same amount of cells was collected via centrifugation. Same buffer was used for each technique of cell lysis in order to get the best protein profiles in terms of band or spot intensity and number. The cells of A. leei were lysed using sonication in ice-cold water bath, sonication with probe and freeze-thawing followed by sonication in iced bath. Conclusion: Nevertheless it can be concluded from the qualitative analysis using SDS-PAGE and 2D gel electrophoresis that freeze thawing mode of cell lysis produced excellent result among others as the protein spots produced were precise with less streaking observed.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	6635/52510
Uncontrolled Keywords:	Alexandrium spp., Protein profiling, Alexandrium leei, SDS Page, 2D Gel electrophoresis
Subjects:	N Fine Arts > ND Painting > ND1288 Special subjects. Human figure. Portraits. Lanscape painting. Marine painting. Animal. Birds. Sports.Flowers. Fruits etc.
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Noraslinda Muhamad Bunnori
Date Deposited:	30 Nov 2016 14:52
Last Modified:	25 Sep 2018 15:27
URI:	http://irep.iium.edu.my/id/eprint/52510

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