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Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient

Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Ramli, Roszaman (2016) Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient. Malaysian Journal of Medical Sciences, 23 (3). pp. 40-48. ISSN 1394-195X

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Abstract

BACKGROUND: Spermatogonial stem cells (SSCs) are classifiedas a unique adult stem cells that have capability to propagate, differentiate, and transmit genetic information to the next generation. Studies on human SSCs may help resolve male infertility problems, especially in azoospermia patients. Therefore, this study aims to propagate SSCs in-vitro with a presence of growth factor and detect SSC-specific protein cell surface markers. METHODS: The sample was derived from non-obstructive azoospermic (NOA) patient. The disassociation of SSCs was done using trypsin. Specific cultures in serum-free media with added basic fibroblast growth factor (bFGF) were developed to support self-renewal division. This undifferentiated protocol was performed for 49 days. Cells were analysed on days 1, 7, 14, 21, and 49. RESULTS: Human SSCs began to aggregate and form colonies after 14 to 21 days in specific culture. Then, the cells were successful expanded and remained stable for a duration of 49 days. Four specifics markers were identified using immunofluorescence in SSCs on day 49: ITGα6, ITGβ CD9, and GFRα1. CONCLUSION: This approach of using in vitro culture with additional growth factor is able to propagate SSCs from non-obstructive azoospermia patient via detection of protein cell surface markers.

Item Type: Article (Journal)
Additional Information: 4508/52198
Uncontrolled Keywords: cell protein marker; in vitro; non-obstructive azoospermia; spermatogonial stem cells
Subjects: R Medicine > RG Gynecology and obstetrics
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Medicine > Department of Obstetric & Gynecology
Kulliyyah of Medicine > IIUM Fertility Centre
Kulliyyah of Nursing
Depositing User: salina mohammad
Date Deposited: 11 Oct 2016 12:19
Last Modified: 21 Dec 2016 08:31
URI: http://irep.iium.edu.my/id/eprint/52198

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