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Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia

Barudin, Mohd Aiman and Mat Yusof, Afzan (2016) Identification of Cryptosporidium in goats by 18S rRNA across four districts in Terengganu, Malaysia. In: Malaysian Society for Microbiology Postgraduate Seminar 2016 (MSMPS 2016), 24 August 2016, Bangi, Selangor. (Unpublished)

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The research aimed to access the first distribution and genotypes of Cryptosporidium spp. using molecular tools in Malaysian goats from four farms in Terengganu. Methodology and results: A total of 454 goat faecal specimens were randomly collected from four selected farms located Kuala Terengganu, Setiu, Marang and Besut districts. Cryptosporidium spp. positive samples from the qualitative examination using combination of modified formalin-ether concentration technique (M-FECT) and modified Ziehl-Neelsen (MZN) staining were withheld for molecular characterization using nested PCR assay targeting the 18S rRNA gene. The 18S rRNA-based PCR successfully identified the predominance of zoonotic species of Cryptosporidium parvum among all studied samples. Conclusion, significance and impact of study: The genotyping study signified that goats should be considered as one of the most important reservoirs for Cryptosporidium parvum in the studied farms. It is suggested that there is the possibility of zoonotic and anthroponotic transmissions of asymptomatic cryptosporidiosis among the goats and animal handlers. Therefore, the need of further molecular subtyping of Cryptosporidium species/genotypes together with comprehensive sanitary monitoring by goat handlers are highly recommended to minimize the transmission of this parasite.

Item Type: Conference or Workshop Item (Other)
Additional Information: 6886/52080
Uncontrolled Keywords: Cryptosporidium, goats
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr. Afzan Mat Yusof
Date Deposited: 05 Oct 2016 08:59
Last Modified: 05 Oct 2016 08:59
URI: http://irep.iium.edu.my/id/eprint/52080

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