Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia

Mat Yusof, Afzan and Kumar, Suresh (2013) Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia. In: Biomicroworld2013 : V International Conference on Environmental , Industrial and Applied Microbiology, 2nd-4th October 2013, Madrid Spain. (Unpublished)

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Abstract

Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previously shown ultrastructural evidence of another asexual mode of reproduction other than binary fission seen in Trichmonas vaginalis. The present study compares the number of multi-nucleated forms isolated from cervical neoplasia (CN) and non-cervical neoplasia (NCN) patients. The present study highlights the detailed characterization of the morphological aspects of 4 CN and 4 NCN isolates by using fixed staining and fluorescent staining of nuclear content. A sequential transformation highlighting nuclear division leading to the formation of multi-nucleated parasite is shown. We observe that this mode of reproduction is not commonly seen and postulate that it could be triggered to increase progeny formation due to stress. These forms were viable when tested with trypan blue dye test. The study confirms that the parasite divides by an asexual mode of reproduction other than binary fission and the parasites isolated from cervical neoplasia showing larger numbers of nuclei accounting for higher parasite numbers in cultures.

Item Type:	Conference or Workshop Item (Other)
Additional Information:	6886/52043
Uncontrolled Keywords:	Trichomonas vaginalis, multinucleated, cervical neoplasia, Malaysia
Subjects:	Q Science > Q Science (General) R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology (including Cancer)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr. Afzan Mat Yusof
Date Deposited:	05 Oct 2016 10:25
Last Modified:	05 Oct 2016 10:25
URI:	http://irep.iium.edu.my/id/eprint/52043

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