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DNA methylation of disrupted in schizophrenia 1 (disc1) gene in schizophrenia using methylight taqman assay

Abd. Rahim, Nour El Huda and Rahim, Mohd Nabil Fikri and Ku Zaifah, Norsidah and Mohd Noor, Hanisah and Abdullah, Kartini and Talib, Norlelawati A. (2016) DNA methylation of disrupted in schizophrenia 1 (disc1) gene in schizophrenia using methylight taqman assay. In: Medical Research Symposium (MRS) and Pasific Partner Partnership (PP16) in conjuction with Kuantan Research Day (KRD) 2016, 4th August 2016, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Significant evidences from functional studies have shown that DISC1 gene has a role in the pathogenesis of schizophrenia, although the basis of the genetic defect has yet to be established. There has been a shift of emphasis from DISC1 gene variations to other genetic defect such as copy number and epigenetic, both of which have not been well investigated. Thus, the aim of this study is to examine the DNA methylation status of DISC1 gene in patients with schizophrenia. Methods: In this study, 239 subjects were included, 117 schizophrenia patients and 122 healthy controls. Genomic DNA was derived from peripheral blood and bisulfite converted. The DNA methylation level was quantitatively measured by Methylight Taqman analysis. Sociodemographic and the clinical parameters were noted. The severity of the clinical symptoms was assessed using Positive and Negative Syndrome Scale (PANSS). Results: The mean age and gender distribution of the study groups were similar. There were no significant differences in the methylation level of DISC1 between the patients and control groups When patients were compared by age, duration of illness, age at diagnosis, body mass index, smoking status, PANSS score, and types of antipsychotic treatment, the DNA methylation level of DISC1, did not show any significant difference (p>0.05). Conclusions: This study found no significant difference in methylation level of DISC1 gene between schizophrenia patients and healthy control. Therefore it is suggested that aberrant DNA methylation of DISC1 most probably does not contribute to the pathogenesis of schizophrenia.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 3616/52015
Uncontrolled Keywords: Schizophrenia, DNA methylation, DISC1
Subjects: R Medicine > R Medicine (General)
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine > Department of Psychiatry
Depositing User: Dr Nour El Huda Abd Rahim
Date Deposited: 28 Feb 2017 11:51
Last Modified: 01 Aug 2017 16:28
URI: http://irep.iium.edu.my/id/eprint/52015

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