Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3

Noor Batcha, Ibrahim Ali and Raja Izzuddin, Raja Hawa Nurathirah and Ahmad, Abdul Aziz and Mohd Salleh, Hamzah (2016) Temperature effect on expression of soluble Endoglucanase I from Fusarium oxysporum using E. coli Lemo21:DE3. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

Formation of inclusion bodies especially when expressing eukaryotic recombinant proteins in prokaryotic host such as E. coli is a challenge. Ability to express soluble proteins in E.coli will enable us to utilize all the benefits of E.coli as a host mainly the short doubling time. As few reports indicates that temperature influences the expression of recombinant proteins, this study aims to observe its effect on solubility of endoglucanase I. The optimum temperature for expressing endoglucanase I from Fusarium oxysporum using Lemo21:DE3 as a host is reported here.

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	3704/51788
Uncontrolled Keywords:	Inclusion bodies, Lemo21:DE3, Fusarium oxysporum.
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Prof. Dr. Ibrahim Ali Noorbatcha
Date Deposited:	24 Aug 2016 15:00
Last Modified:	24 Aug 2016 15:00
URI:	http://irep.iium.edu.my/id/eprint/51788

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