Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product

Omar, Muhammad Nor and Mohd Hasali, Nor Hazwani and Yarmo, Mohd Ambar (2016) Biotransformation of ethyl p-methoxycinnamate and cytotoxicity activity of its biotransformed product. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

The extraction of Kaempferia galanga rhizome using steam distillation and supercritical fluid extraction (SFE) was carried out. After fractionation, the major compound of the K. galanga extract, ethyl p-methoxycinnamate (EPMC) was transformed using Aspergillus niger into ethyl p-hydroxycinnamate (EPHC). The biological anticancer activity of EPMC and its biotransformed product (EPHC) was established by cytotoxicity activity on the human breast cancer (MCF-7) cell line using MTT assay. Ethyl p-hydroxycinnamate (EPHC) was most cytotoxic against MCF-7 at 1000 μg/mL where percentage of cell viability was 9.87 %, while IC50 was 340 μg/mL. EPHC showed slightly higher cytotoxicity activity compared to EPMC. The results of this study show that the biotransformation process was able to produce a metabolite (EPHC) with higher cytotoxicity activity compared to its parent compound (EPMC).

Item Type:	Conference or Workshop Item (Invited Papers)
Additional Information:	5626/51783
Uncontrolled Keywords:	Kaempferia galanga; ethyl p-methoxycinnamate; biotransformation; cytotoxic
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science
Depositing User:	Prof Dr Muhammad Nor Omar
Date Deposited:	24 Aug 2016 11:02
Last Modified:	24 Aug 2016 11:02
URI:	http://irep.iium.edu.my/id/eprint/51783

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