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Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration

Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa’zamuddin and Hashim, Rosyafirah and Mat Nawi, Nur Farhana and Zulkifly, Ahmad Hafiz and Sha’ban, Munirah (2016) Characterization of poly (lactic-co-glycolic acid) / atelocollagen hybrid scaffold for intervertebral disc regeneration. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

The objective of the present study is to characterize PLGA and atelocollagen type 2 (PLGA-A) hybrid scaffolds in terms of swelling capacity, thermal properties and mechanical strength. PLGA without atelocollagen was used as control.Porous PLGA scaffold is fabricated via salt leaching/ solvent-casting using salt at 350-400 micron. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-Hydroxysuccinimide (NHS) were used to crosslink atelocollagen with PLGA. The incorporation of atelocollagen and PLGA is confirmed by the presence of amide formation of amide bonds at 1650cm-1 using Fourier transform infrared spectroscopy (ATR-FTIR). Scanning Electron Microscopy (SEM) images demonstrated addition of atelocollagen did not jeopardize porous structure of PLGA scaffolds. Differential Scanning Calorimetry (DSC) analysis showed increment of glass transition, Tg of PLGA-A by 13°C. The swelling ratio of PLGA-A scaffold showed 4.8 times increase water uptake within 24-hour period. The mechanical strength of PLGA-A is enhanced by 255.95% when tested with Instron E3000 machine. These findings suggest PLGA incorporation with atelocollagen type 2 may become a promising biomaterial for intervertebral disc regeneration.

Item Type: Conference or Workshop Item (Invited Papers)
Additional Information: 2397/51690
Uncontrolled Keywords: PLGA, Atelocollagen, Swelling ratio, Mechanical Strength, Glass Transition, AT-FTIR
Subjects: R Medicine > R Medicine (General)
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User: Prof Dr Ahmad Hafiz Zulkifly
Date Deposited: 19 Aug 2016 08:49
Last Modified: 19 Aug 2016 08:49
URI: http://irep.iium.edu.my/id/eprint/51690

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