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Exploring the feasibility in using medical computerized tomography to monitor growth progression in tissue engineered construct prepared from cells seeded on Poly(Lactic-co-Glycolic Acid) based scaffolds

Mat Nawi, Nur Farhana and Zainuddin, Zainul Ibrahim and Md Nazir, Noorhidayah and Mohamad, Mohd Yusof and Ahmad Radzi, Muhammad Aa’zamuddin and Hashim, Rosyafirah and Sha’ban, Munirah (2016) Exploring the feasibility in using medical computerized tomography to monitor growth progression in tissue engineered construct prepared from cells seeded on Poly(Lactic-co-Glycolic Acid) based scaffolds. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Abstract

This study attempted to examine the feasibility of using medical Computerized Tomography (CT) imaging scanner to monitor the growth of tissue engineered constructs (TECs) in vitro. A preliminary CT examination was performed on TECs prepared from serially expanded chondrocytes seeded on poly (lactic-co-glycolic acid) (PLGA) based scaffolds that acted as cell carriers. One day old PLGA with fibrin and cells (PFC), PLGA and cells (PC) as well as PLGA with Fibrin (PF) constructs were stored in normal saline prior to CT examination. The kVp used was 80 Kvpwhile the effective mAs was 11 mAs. Sharp kernel was chosen as the reconstruction parameters and slice thickness used was 0.1mm.A PLGA without cells or fibrin was used as control. X-ray attenuation of TECs, measured in Hounsfield units (HU) or, also known as CT values were observed. The results indicated that the control PLGA, the PFC, the PC and also the PF constructs gave CT values between -665HU to 477HU, between 149HU to 181HU, between -120HU to 263HU and -209HU to 185HU respectively. These initial results indicate that the x-ray absorption-based quantitative differentiation between TECs could be appreciated. The CT values documented suggest that the differences in attenuation values are dependent on the condition of the scaffolds or the TECs themselves. Furthermore, the use of intravascular ultrasound (IVUS) software installed in the CT system was found to be useful in appreciating the different compositions within the scanned samples. It is hoped that the outcomes of this preliminary study can serve as a baseline for future research to look for an alternative method to quantitatively monitor the growth of TECs in vitro as well as in vivo.

Item Type: Conference or Workshop Item (Invited Papers)
Additional Information: 3657/51668
Uncontrolled Keywords: tissue engineering; medical imaging; CT scan; cell culture; scaffold; biomaterials; attenuation; Poly(Lactic-co-Glycolic Acid); articular cartilage; growth progression
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences > Department of Diagnostic Imaging and Radiotherapy
Depositing User: Zainul Ibrahim Zainuddin
Date Deposited: 18 Aug 2016 09:05
Last Modified: 18 Aug 2016 09:40
URI: http://irep.iium.edu.my/id/eprint/51668

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