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Statistical optimization of gelatin immobilization to improve PCL microcarrier surface

Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis and Jimat, Dzun Noraini (2016) Statistical optimization of gelatin immobilization to improve PCL microcarrier surface. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur.

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Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, oxygen functional group was introduced via ultraviolet irradiation and ozone aeration (UV/O3) system to allow covalent immobilization of gelatin on the PCL microcarrier surface using zero-length crosslinker reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide (EDAC/NHS). The optimum conditions for maximizing the amount of gelatin on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10mM NHS concentration and, 80 mg/ml gelatin.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 3774/51646 (ISBN: 978-983-42978-8-6)
Uncontrolled Keywords: microcarrier; gelatin immobilization; polycaprolactone; optimization
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Date Deposited: 17 Aug 2016 11:03
Last Modified: 19 Oct 2017 14:41
URI: http://irep.iium.edu.my/id/eprint/51646

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