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Fabrication of Fucoxanthin-Loaded Microsphere ( F-LM) by two steps double-emulsion solvent evaporation methods and characterization of fucoxanthin before and after microencapsulation

Noviendri, Dedi and Jaswir, Irwandi and Bakhtiar, M. Taher and Mohamed, Farahidah and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali and Octavianti, Fitri and Lestari, Widya and Hendri, Ridar and Ahmad, Hasna and Miyashita, Kazuo and Abdullah, Alias (2016) Fabrication of Fucoxanthin-Loaded Microsphere ( F-LM) by two steps double-emulsion solvent evaporation methods and characterization of fucoxanthin before and after microencapsulation. Journal of Oleo Science, 65 (8). pp. 641-653. ISSN 1347-3352

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Abstract

Microencapsulation is a promising approach in drug delivery to protect the drug from degradation and allow controlled release of the drug in the body. Fucoxanthin-loaded microsphere (F-LM) was fabricated by two step w/o/w double emulsion solvent evaporation method with poly (L-lactic-co-glycolic acid) (PLGA) as carrier. The effect of four types of surfactants (PVA, Tween-20, Span-20 and SDS), homogenization speed, and concentration of PLGA polymer and surfactant (PVA), respectively, on particle size and morphology of F-LM were investigated. Among the surfactants tested, PVA showed the best results with smallest particle size (9.18 µm) and a smooth spherical surface. Increasing the homogenization speed resulted in a smaller mean F-LM particle size [d(0.50)] from 17.12 to 9.18 µm. Best particle size results and good morphology were attained at homogenization speed of 20 500 rpm. Meanwhile, increased PLGA concentration from 1.5 to 11.0 (% w/v) resulted in increased F-LM particle size. The mean particle size [d(0.5)] of F-LM increased from 3.93 to 11.88 µm. At 6.0 (% w/v) PLGA, F-LM showed the best structure and external morphology. Finally, increasing PVA concentration from 0.5 to 3.5 (% w/v) resulted in decreased particle size from 9.18 to 4.86 µm. Fucoxanthin characterization before and after microencapsulation was carried out to assess the success of the microencapsulation procedure. Thermo gravimetry analysis (TGA), glass transition (Tg) temperature of F-LM and fucoxanthin measured using DSC, ATR-FTIR and XRD indicated that fucoxanthin was successfully encapsulated into the PLGA matrix, while maintaining the structural and chemical integrity of fucoxanthin.

Item Type: Article (Journal)
Additional Information: 5784/51371
Uncontrolled Keywords: fucoxanthin, microsphere, microencapsulation, w/o/w double emulsion, solvent evaporation method
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes: International Institute for Halal Research and Training (INHART)
Kulliyyah of Architecture and Environmental Design
Kulliyyah of Dentistry
Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Widya Lestari
Date Deposited: 01 Aug 2016 02:59
Last Modified: 19 Apr 2017 07:58
URI: http://irep.iium.edu.my/id/eprint/51371

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