Screening of factors affecting the enzymatic hydrolysis of empty fruit bunches in aqueous ionic liquid and locally produced cellulase system

Alam, Md Zahangir and Elgharbawy, Amal A. and Moniruzzaman, Muhammad and Kabbashi, Nassereldeen A. and Jamal, Parveen (2016) Screening of factors affecting the enzymatic hydrolysis of empty fruit bunches in aqueous ionic liquid and locally produced cellulase system. International Journal of Chemical, Molecular, Nuclear, Materials and Metallurgical Engineering, 10 (7). pp. 843-847.

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Abstract

The enzymatic hydrolysis of lignocellulosic biomass is one of the obstacles in the process of sugar production, due to the presence of lignin that protects the cellulose molecules against cellulases. Although the pretreatment of lignocellulose in ionic liquid (IL) system has been receiving a lot of interest; however, it requires IL removal with an anti-solvent in order to proceed with the enzymatic hydrolysis. At this point, introducing a compatible cellulase enzyme seems more efficient in this process. A cellulase enzyme that was produced by Trichoderma reesei on palm kernel cake (PKC) exhibited a promising stability in several ILs. The enzyme called PKC-Cel was tested for its optimum pH and temperature as well as its molecular weight. One among evaluated ILs, 1,3-diethylimidazolium dimethyl phosphate [DEMIM] DMP was applied in this study. Evaluation of six factors was executed in Stat-Ease Design Expert V.9, definitive screening design, which are IL/buffer ratio, temperature, hydrolysis retention time, biomass loading, cellulase loading and empty fruit bunches (EFB) particle size. According to the obtained data, IL-enzyme system shows the highest sugar concentration at 70 °C, 27 hours, 10% IL-buffer, 35% biomass loading, 60 Units/g cellulase and 200 μm particle size. As concluded from the obtained data, not only the PKC-Cel was stable in the presence of the IL, also it was actually stable at a higher temperature than its optimum one. The reducing sugar obtained was 53.468±4.58g/L which was equivalent to 0.3055 g reducing sugar/g EFB. This approach opens an insight for more studies in order to understand the actual effect of ILs on cellulases and their interactions in the aqueous system. It could also benefit in an efficient production of bioethanol from lignocellulosic biomass.

Item Type:	Article (Journal)
Additional Information:	4157/51239
Uncontrolled Keywords:	Cellulase, hydrolysis, lignocellulose, pretreatment, stability.
Subjects:	T Technology > TD Environmental technology. Sanitary engineering > TD194 Environmental effects of industries and plants
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Dr Nassereldeen Kabbashi
Date Deposited:	21 Jul 2016 14:38
Last Modified:	17 Apr 2019 09:37
URI:	http://irep.iium.edu.my/id/eprint/51239

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