Simulation of absorption spectrum of photosynthetic pigments of chlorella vulgaris B algae

Mahmoud Ahmed, Gharib Subhi and Hassan, Taher and Othman, Rashidi and Messikh Azeddine, Azeddine (2016) Simulation of absorption spectrum of photosynthetic pigments of chlorella vulgaris B algae. Malaysian Journal of Mathematical Sciences, Volume 10 (S) (Special Issue- Part I). pp. 123-130. ISSN 1823-8343 E-ISSN 2289-750X

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Abstract

Absorption spectrum of Chlorella vulgaris B algae was reconstructed by simulated the absorption spectrum of the mixture under examination by a linear combination of the absorption spectra of all its constituents. A C30 reversed high performance liquid chromatography equipped with photodiode array detector (HPLC-PAD) and absorption spectrophotometer were used to separate and identify photosynthetic pigments of Chlorella vulgaris B. Spectrum-reconstruction method (SRCM) was used to calculate concentrations of total carotenoids, chlorophylls a and b. Absorption spectrum of the total pigments extracted of Chlorella vulgaris B. was reconstructed from the sum of the spectrum of individual extracted pigments taken from (HPLC-PAD), and compared using normalization with absorption spectrum of the total pigments recorded by spectrophotometer. A total of seven Pigments were separated and identified

Item Type:	Article (Journal)
Additional Information:	5541/50717
Uncontrolled Keywords:	Chlorella Vulgaris; Pigment Composition; Multi-Component Analysis; HPLC; Absorption Spectrum
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Architecture and Environmental Design Kulliyyah of Engineering Kulliyyah of Information and Communication Technology Kulliyyah of Information and Communication Technology
Depositing User:	Dr Gharib Mahmoud
Date Deposited:	14 Mar 2017 11:05
Last Modified:	14 Mar 2017 11:15
URI:	http://irep.iium.edu.my/id/eprint/50717

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