Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line

Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2014) Cellular uptake of Nigella sativa oil-PLGA microparticle by PC12 cell line. Journal of Microencapsulation, 31 (6). pp. 600-608. ISSN 0265-2048 E-ISSN 1464-5246

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Abstract

The aim of this study is to investigate the cell uptake of Nigella sativa oil (NSO)-PLGA microparticle by neuron-like PC-12 cells in comparison to surfactants; hydrophilic (Tween 80 & Triton X100) and hydrophobic (Span 80). Solvent evaporation was used to precisely control the size, zeta potential and morphology of the particle. The results revealed varying efficiencies of the cell uptake by PC-12 cells, which may be partially attributed to the surface hydrophobicity of the microparticles. Interestingly, the uptake efficiency of PC-12 cells was higher with the more hydrophilic microparticle. NSO microparticle showed evidence of being preferably internalised by mitotic cells. Tween 80 microparticle showed the highest cell uptake efficiency with a concentration-dependent pattern suggesting its use as uptake enhancer for nonscavenging cells. In conclusion, PC-12 cells can take up NSO-PLGA microparticle which may have potential in the treatment of neurodegenerative disease.

Item Type:	Article (Journal)
Additional Information:	4341/49758
Uncontrolled Keywords:	Hydrophobicity, microparticles, neurodegenerative, Nigella sativa oil, PLGA, surfactant, uptake
Subjects:	R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy
Depositing User:	Assoc Prof Farahidah Mohamed
Date Deposited:	05 Apr 2016 14:18
Last Modified:	05 Jan 2018 11:27
URI:	http://irep.iium.edu.my/id/eprint/49758

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