Rapid investigation of α-glucosidase inhibitory activity of Clinacanthus nutans leaf using infrared fingerprinting

Murugesu, Suganya and Ahmed, Qamar Uddin and Uzir, Bisha Fathamah and Nik Yusof, Nik Idris and Perumal, Vikneswari and Ibrahim, Zalikha and Abas, Faridah and Shaari, Khozirah and Khatib, Alfi (2019) Rapid investigation of α-glucosidase inhibitory activity of Clinacanthus nutans leaf using infrared fingerprinting. Vibrational Spectroscopy, 100. pp. 22-29. ISSN 0924-2031 E-ISSN 1873-3697

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Abstract

The analytical method used in the quality control of Clinacanthus nutans leaves has not been well developed. Therefore, this study aimed to develop a simple analytical method to predict α-glucosidase inhibitory activity of this herb based on its infrared fingerprinting. The dried extracts obtained from maceration using solvents with different polarities were evaluated for the α-glucosidase inhibitory activity and analysed through infrared spectroscopy. Multivariate data analysis was performed by correlating the bioactivity and infrared spectrum of each extract using partial least square method. The loading plot from multivariate data analysis revealed that CH and CO infrared signals from terpenoids in the extract were positively correlated with the α-glucosidase inhibitory activity. The developed partial least square model was validated through a testing on the external samples. The result concludes that the developed model is valid and capable of predicting α-glucosidase inhibitory activity of the external samples.

Item Type:	Article (Journal)
Additional Information:	4921/49730
Uncontrolled Keywords:	Clinacanthus nutansα-GlucosidasePartial least squareFingerprintingInfrared spectroscopyChemometrics
Subjects:	R Medicine > RS Pharmacy and materia medica R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	19 Mar 2019 16:30
Last Modified:	12 Jul 2019 15:13
URI:	http://irep.iium.edu.my/id/eprint/49730

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