Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times

Yusof, Nur A'thifah and Isha, Azizul and Ismail, Intan Safinar and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Rukayadi, Yaya (2015) Infrared–metabolomics approach in detecting changes in andrographis paniculata metabolites due to different harvesting ages and times. Journal of the Science of Food and Agriculture, 95 (12). pp. 2533-2543. ISSN 0022-5142 E-ISSN 1097-0010

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Abstract

BACKGROUND: The metabolite changes in three germplasm accessions of Malaysia Andrographis paniculata (Burm. F.) Nees, viz. 11265 (H), 11341 (P) and 11248 (T), due to their different harvesting ages and times were successfully evaluated by attenuated total reflectance (ATR)–Fourier transform infrared (FTIR) spectroscopy and translated through multivariate data analysis of principal component analysis (PCA) and orthogonal partial least square–discriminant analysis (OPLS-DA). This present study revealed the feasibility of ATR-FTIR in detecting the trend changes of the major metabolites – andrographolide and neoandrographolide – functional groups in A. paniculata leaves of different accessions. The harvesting parameter was set at three different ages of 120, 150 and 180 days after transplanting (DAT) and at two different time sessions of morning (7:30–10:30 am) and evening (2:30–5.30 pm). RESULTS: OPLS-DA successfully discriminated the A. paniculata crude extracts into groups of which the main constituents – andrographolide and neoandrographolide – could be mainly observed in the morning session of 120 DAT for P and T, while H gave the highest intensities of these constituents at 150 DAT. CONCLUSION: The information extracted from ATR-FTIR data through OPLS-DA could be useful in tailoring this plant harvest stage in relation to the content of its two major diterpene lactones: andrographolide and neoandrographolide.

Item Type:	Article (Journal)
Additional Information:	6915/49725
Uncontrolled Keywords:	: Fourier transform infrared; metabolomics; Andrographis paniculata; harvesting age; harvesting time
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	19 Feb 2016 15:40
Last Modified:	21 Nov 2017 16:01
URI:	http://irep.iium.edu.my/id/eprint/49725

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