Pencirian dan aktiviti antibakteria endofit Streptomyces sp. dari Hutan Simpan Penyelidikan UKM Bangi

Mohamad Zin, Noraziah and Abu Bakar, Nur Faizah and Ismail, Aishah and Baba, Mohd Shukri and Sidek, Nik Marzuki (2015) Pencirian dan aktiviti antibakteria endofit Streptomyces sp. dari Hutan Simpan Penyelidikan UKM Bangi. Malaysian Applied Biology, 44 (1). pp. 107-117. ISSN 0126-8643

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Abstract

Endofit yang bersimbiosis dengan tumbuhan merupakan mikroorganisma yang terdapat dalam satu sistem jaringan tumbuhan seperti pada akar, daun, batang atau ranting. Ianya dapat menghasilkan metabolit sekunder yang berpotensi untuk digunakan dalam bidang kesihatan sebagai sumber penghasilan antibiotik. Kajian ini bertujuan memencilkan endofit Streptomyces sp. daripada pelbagai tumbuhan ubatan endemik. Kesemua pencilan telah dikenalpasti melalui pemerhatian secara makroskopik dan mikroskopik. Sebanyak 28 pencilan (32%) yang bercirikan Streptomyces sp. telah berjaya dipencilkan menggunakan media pemencil ISP2. Kesemua pencilan adalah bergram positif, mempunyai filamen bercabang serta berbau tanah yang merupakan ciri penting Streptomyces sp. Daripada pemerhatian secara makroskopik, didapati kebanyakan koloni yang terbentuk mempunyai permukaan yang tidak rata serta bersifat kering berkapur. Warna miselium udara pencilan adalah berbeza iaitu kelabu, putih kuning, putih serta hijau kelabu. Selain itu, warna miselium substrat ialah putih kuning, coklat, jingga tua, hijau zaitun dan kelabu muda. Amat menarik kerana terdapat beberapa pencilan yang berbeza cirinya telah dipencilkan daripada pokok yang sama. Selain dari itu, terdapat beberapa pencilan yang mempunyai aktiviti antibakteria yang memberikan perencatan 100% terhadap bakteria ujian. Symbiotic endophytic microorganisms to the plant can be found in a network system of plant roots, leaves, stems or twigs. Its produced secondary metabolites which are potential sources in the health field as antibiotics producer. The aim of this study is to isolate endophytic Streptomyces sp. from various medicinal plants in an endemic area. All isolates were identified through macroscopic and microscope observation. A total of 28 isolates (32%) with Streptomyces sp. characterization were successfully isolated using a specific media, ISP2. All strains are gram positive with branched filament and earthy essential odors which are characteristic of Streptomyces sp. From macroscopic observation, colonies formed mostly uneven and dry calcareous. Aerial mycelium of isolates showed different colors which were gray, white, yellowish, and grayish green. Nevertheless, the substrate mycelium showed white yellowish, brown, dark orange, olive green and light gray in color. It is interesting that some distinct characteristics of isolates were isolated from the same plant. Apart from that, numbers of isolate have the antibacterial activity with 100% inhibition against test bacteria.

Item Type:	Article (Journal)
Additional Information:	7342/48774
Uncontrolled Keywords:	Endofit, Streptomyces, antimikrob Endophytes, Streptomyces, antimicrobes
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Mohd Shukri Baba
Date Deposited:	23 Mar 2016 09:08
Last Modified:	07 Aug 2017 15:38
URI:	http://irep.iium.edu.my/id/eprint/48774

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