Antibiotic-Nigella Sativa Fusion (ANF) as a novel approach intended for the treatment against bacteria forming biofilm in osteomyelitis

Mohamed, Farahidah and Mohd Shafri, Mohd Affendi and Mohd Yusof, Nazri and Harun Ismail, Ahmad Fahmi and Yaacob, Khairul Ikhwan and Yusof, Fatin Athirah and Shahdan, Intan Azura (2015) Antibiotic-Nigella Sativa Fusion (ANF) as a novel approach intended for the treatment against bacteria forming biofilm in osteomyelitis. In: Eurobiofilms 2015: Fourth European Congress on Microbial Biofilms, 23rd-26th June 2015, Brno, Czech Republic.

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Abstract

Multi-drug resistant bacteria are one of the global problems facing humanity. One of the bacteria of concern causing osteomylitis is Staphylococcus aureus. Biofilm from this bacterium in the infected bones has been clinically isolated from the infected patients. A novel formulation of a contemporary antibiotic i.e Gentamicin (an Aminoglycoside) was fused with prophetic medicine i.e Nigella Sativa oil (NSO) (also known as black seed oil) and thereafter characterised. The fusion was done by preparing the emulsions of the two materials with the aid of varying amount of surfactants. Compatibility testing of the two compositions was also conducted pre and post fusion and pre and post gamma irradiation by Fourier-Transform Infra Red (FTIR), HPLC and Thin Layer Chromatography (TLC). In-vitro efficacy test of the fusion was further tested against both the sensitive and the resistant of the commercial and the clinically isolated S.Aureus. Cell viability was also performed on osteoclast cells to determine preliminary safety profile of the fusion. Results indicate that the fusions are physically and chemically compatible and stable with significantly higher safety level than gentamicin alone. The fusions showed ability to inhibit biofilm formation and are also more effective in-vitro particularly against the resistant S.Aureus unlike Gentamicin. With further clinical proofs, the findings could have high impact on current treatment regimen against multi-drug resistant bacteria particularly for osteomyelitis. It is also pave the way for more antibiotics to be fused with NSO and perhaps other prophetic medicine and tested for the same purpose or others.

Item Type:	Conference or Workshop Item (Abstract)
Additional Information:	4341/48736
Uncontrolled Keywords:	Antibiotic-Nigella Sativa Fusion (ANF)
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Mohd Affendi Mohd Shafri
Date Deposited:	18 Feb 2016 09:25
Last Modified:	18 Feb 2016 09:25
URI:	http://irep.iium.edu.my/id/eprint/48736

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