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Performance of β-glucosidase immobilized on calcium alginate beads

Jameel, Ahmed Tariq and Yusof, Faridah and Johana, Syaira (2013) Performance of β-glucosidase immobilized on calcium alginate beads. In: The Third International Conference on BIOTECHNOLOGY ENGINEERING ICBioE’13, July 2-4, 2013, Kuala Lumpur, Malaysia.

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Abstract

Performance of immobilized β-glucosidase obtained from almonds (EC 3.2.1.21) on calcium alginate beads was studied by measuring the activity of the enzyme in terms of the generation of pnitrophenol from the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPG).The immobilized enzyme activity was compared with soluble enzyme and was found to decrease by 36.6% albeit with an increased operational stability in terms of easy recovery from the finished product, recurrent use and scale-up in various reactor configurations. The hydrolysis rate data exhibit Michaelis-Menten kinetics. The Michaelis constant were determined using Langmuir linearized plot which obtained vmax and Km as 20.88 μmol/mL.min and 0.0125 mol/L respectively. However, the immobilized β-Glucosidase did not show a sufficiently good operational stability on reuse. Keywords: Immobilized enzyme, enzyme activity, Michaelis-Menten kinetics, β-glucosidase, pnitrophenyl- β-D-glucopyranoside (PNPG), alginate gel

Item Type: Conference or Workshop Item (Slide Presentation)
Additional Information: 5642/48525
Uncontrolled Keywords: Immobilized enzyme, enzyme activity, Michaelis-Menten kinetics, β-glucosidase, pnitrophenyl-β-D-glucopyranoside (PNPG), alginate gel
Subjects: T Technology > TP Chemical technology
T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Engineering
Depositing User: Dr. Ahmad Tariq Jameel
Date Deposited: 23 Feb 2016 11:29
Last Modified: 06 May 2018 02:09
URI: http://irep.iium.edu.my/id/eprint/48525

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