Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair

Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Md Nazir, Noorhidayah and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Sha'ban, Munirah (2015) Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair. Jurnal Teknologi, 77 (25). pp. 77-82. ISSN 0127–9696 E-ISSN 2180–3722

PDF (Evaluation of three dimensional construct engineered from poly(Lactic-co-glycolic acid)/fibrin hybrid scaffold using rabbit bone marrow mesenchymal stem cells for osteochondral defect repair) - Published Version Restricted to Repository staff only Download (457kB) | Request a copy

Abstract

Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. The engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and more intense expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair.

Item Type:	Article (Journal)
Additional Information:	4928/47426 Corresponding author: Dr. Munirah Sha'ban (Staff no. 6006)
Uncontrolled Keywords:	Tissue engineering, poly(lactic-co-glycolic acid), fibrin, bone marrow mesenchymal stem cells, cartilage
Subjects:	UNSPECIFIED
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User:	Yusnizar Fuad
Date Deposited:	05 Jan 2016 15:57
Last Modified:	15 Jul 2016 10:09
URI:	http://irep.iium.edu.my/id/eprint/47426

Actions (login required)

View Item