DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene

K., Kamarudin and Mohamed Rehan, Maryam and Ramly, Nur Zazarina and Mohamed Rehan, Aisyah (2015) DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene. In: Kolokium Siswazah Sains dan Teknologi (KoSiST'15), 13th Oct. 2015, Akademi Kepimpinan Pengajian Tinggi (AKEPT), Bandar Enstek, Negeri Sembilan.

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Abstract

This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene. Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more specimens and different molecular approaches are required to produce better conclusive results, the current findings gave a better insight of the importance of morphological approach and 16S mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial species standard or reference samples in determining the species identities of processed sea cucumbers from Malaysian markets.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4469/47379
Uncontrolled Keywords:	Stichopus horrens, Holothuria leucospilota, Pangkor Island, 16S mitochondrial ribosomal RNA gene, phylogenetic analyses
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Mrs Aisyah Mohamed Rehan
Date Deposited:	21 Jan 2016 16:07
Last Modified:	24 May 2016 13:26
URI:	http://irep.iium.edu.my/id/eprint/47379

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