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Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice

Abdul Ghani, Radiah and Nik Abdul Malek, Nik Nurasyikin and Md Yusof, Najwa Farhah and Jamil, Elyna Fatini (2015) Ornithine decarboxylase gene expression in human lung adenocarcinoma cell (A549) treated with pomegranate juice. Jurnal Teknologi, 77 (25). pp. 95-99. ISSN 0127–9696 E-ISSN 2180–3722

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Abstract

The polyamine biosynthesis pathway plays a significant role in cell growth, both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. The inhibition of growth was determined using Trypan Blue exclusion and the changes in intracellular polyamine in pomegranate treated cells was observed using High Performance Liquid Chromatography (HPLC). It was found that there was inhibition of A549 cell growth and reduced in intracellular polyamine content in pomegranate treated cells. The ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.

Item Type: Article (Journal)
Additional Information: 4458/47360
Uncontrolled Keywords: Polyamines, Ornithine decarboxylase (ODC), A549, chemoprevention, natural product
Subjects: UNSPECIFIED
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Radiah Abdul Ghani
Date Deposited: 04 Jan 2016 18:06
Last Modified: 15 Jul 2016 09:32
URI: http://irep.iium.edu.my/id/eprint/47360

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