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Morphological change of shed and the effect of VEGF on amniotic membrane scaffold

Md Hashim , SN and Yusof , MFH and Alshehadat , SA and Kannan , TP and Suzina , SAH and Mokhtar, Khairani Idah and Azlina , A (2015) Morphological change of shed and the effect of VEGF on amniotic membrane scaffold. Malaysian Journal of Microscopy, 11. pp. 1-6. ISSN 1823-7010

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Abstract

Angiogenicity is one of the essential components to enable tissue function. It is important to develop a construct that would help in catering oxygen and nutrient to the engineered tissue area. Thus, this study aims to investigate the attachment, spreading and growth of stem cells from human exfoliated deciduous teeth (SHED) on human AM (HAM) with or without vascular endothelial growth factor (VEGF) using scanning electron microscope (SEM), and indirectly see the potential of the HAM as a scaffold to promote angiogenic micro-environment. Since day 1, there were continuous changes of the cell morphology until day 28, SHED treated with VEGF seemed to change its shape from fibroblast-like into a round-shape cell, similar structure as an endothelial-like cell. The structures of filopodia-like were also observed on the treated SHED. SHED without VEGF treatment showed only normal morphological growth on HAM. VEGF is a protein produced to stimulate angiogenesis, and is believed to contribute to the morphological changes of SHED seeded on HAM. This indicates that HAM could be used as a scaffold to allow SHED differentiation into endothelial-like cells with the induction of VEGF.

Item Type: Article (Journal)
Additional Information: 7172/46913
Uncontrolled Keywords: amniotic membrane, vascular endothelial growth factor, stem cells from human exfoliated deciduous teeth
Subjects: R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Dentistry
Depositing User: Assoc Prof Khairani Idah Mokhtar
Date Deposited: 31 Dec 2015 09:39
Last Modified: 13 Dec 2017 18:02
URI: http://irep.iium.edu.my/id/eprint/46913

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