Screening of various parts of phaleria macrocarpa plant for α-glucosidase inhibitory activity

Evan Prince, Sabina and Sarker, Md. Zaidul Islam and Ghafoor, Kashif and Md. Jaffri, Juliana and Ferdosh, Sahena and Elfadıl Elfadl, Babiker and Perumal, Vikneswari and Hamed, Mohamed Bey Baba and Amid, Mehrnoush and Khatib, Alfi (2016) Screening of various parts of phaleria macrocarpa plant for α-glucosidase inhibitory activity. Journal of Food Biochemistry, 40 (2). pp. 201-210. ISSN 1745-4514

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Abstract

Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α -glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa . Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6±0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC500.8±0.1μg/mL). The fruit flesh (IC501.3±0.2μg/mL)and leaves (IC501.6±0.6μg/mL) had also well effectively. The identified metabo-lites are predominantly phenolics, carbohydrates, triterpenes and organic acids,such as D-fructose, squalene,α -linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus,P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus.

Item Type:	Article (Journal)
Additional Information:	6605/46843
Uncontrolled Keywords:	Alpha linolenic acids; Anti-hyperglycemic activity; Chemical profiling; Gas chromatography-mass spectrometries (GC-MS); Hydrolyzing enzymes; Inhibitory activity; Similarity indices; Type 2 diabetes mellitus
Subjects:	Q Science > Q Science (General) T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Technology Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr. Sahena Ferdosh
Date Deposited:	06 Jan 2016 19:54
Last Modified:	13 Feb 2017 11:07
URI:	http://irep.iium.edu.my/id/eprint/46843

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