Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia

Zainal Abidin, Zaima Azira and Abdul Malek, Nurfathiah and Zainuddin, Zarina and Khan Chowdhury, Ahmed Jalal (2016) Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia. Frontiers in Life Science, Vol. 9 (1). pp. 24-31. ISSN 2155-3769 E-ISSN 2155-3777

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Abstract

This study aimed to isolate and evaluate the antagonistic potential of actinomycetes from mangrove forest of Pahang, Malaysia. Sediment samples from seven different sites were pretreated using wet heat and dry heat methods followed by dilution plating on eight isolation media. In total, 4850 isolates were successfully recovered, with inorganic salt–starch agar displaying the highest percentage of recovery (31.7%), followed by actinomycete isolation agar (24.1%). The wet heat pretreatment was found to be more effective in the enumeration of actinomycetes, since 81.4% of total isolates were yielded using this technique, whereas dry heat treatment was better in the enumeration of spore-forming actinomycetes. After both pretreatments, Streptomyces-like and Micromonospora-like isolates were the most predominant. The antagonistic activities of the representative isolates were evaluated using the cross-streak method. In total, 78 isolates were active against at least one of the test organisms. Among them, 28.2% exhibited antibacterial activity, 23.1% exhibited antifungal activity and 48.7% displayed both. Nine isolates demonstrated broad antagonism by inhibiting the growth of all test organisms. The presence of a relatively large number of bioactive isolates suggests that Pahang mangrove forest is a potential source of actinomycetes with biosynthetic capabilities

Item Type:	Article (Journal)
Additional Information:	4416/46403
Uncontrolled Keywords:	mangrove actinomycetes; Pahang; pretreatments; selective isolation; antagonistic activity
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	zaima azira zainal abidin
Date Deposited:	30 Dec 2015 11:54
Last Modified:	08 Feb 2017 10:00
URI:	http://irep.iium.edu.my/id/eprint/46403

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• Selective isolation and antagonistic activity of actinomycetes from mangrove forest of Pahang, Malaysia. (deposited 30 Dec 2015 11:54) [Currently Displayed]
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