Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy

Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2015) Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy. In: Controlled Released & Delivery Symposium 2015 (CRDDS2015), 15th-16th Aug. 2015, Faculty of Pharmacy, Universiti Kebangsaan Malaysia. (Unpublished)

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Abstract

Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy.

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	7245/46387
Uncontrolled Keywords:	CTAB, Chitosan
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User:	Dr. Abd Almonem Doolaanea
Date Deposited:	27 May 2016 10:43
Last Modified:	27 May 2016 10:43
URI:	http://irep.iium.edu.my/id/eprint/46387

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