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Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma

Mohammad, Mardhiah and Mat Yusof, Afzan and Kaja Mohideen, Syahirah and Syed Bahrom, Sharifah Norbaizura and Roshdi, Ridhwan and Parman, Adibah (2015) Investigation on the difference expression of type IV collagen α1(IV)- α6(IV) chain mrna in normal fibroblast and in skin cell malignant melanoma. Jurnal Teknologi, 77 (25). pp. 141-145. ISSN 0127–9696 E-ISSN 2180–3722

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Abstract

Collagen IV is the major basement membrane protein that influences adhesion, proliferation, and migration of cells. The collagen composed of a network chains a1 to a6. The characterization of this collagen IV will correlates the relationship of collagen gene expression and cancer. This is important in order to provide more detailed understanding of the expression of collagen in tumor cells. . The aim of this study is to determine the a1 to a6 (IV) mRNA expression in the cell lines obtained from skin and melanoma cell. To investigate the mRNA expression, the RNA was extracted from the fibroblast and melanoma (A375) cell lines. The RNA was subjected to reverse transcription and then synthesized. The mRNA expression levels were measured using real time PCR with related to internal control, GAPDH. The study identified that a1, a2, a4, a5 and a6 of the a1-a6 (IV) were expressed in skin fibroblast. This corresponds to the a1a1a2 and a5a5a6 networks. However, in melanoma cell lines the collagen IV a2, a4, a5 and a6 mRNA was observed in low level compared to a1 and this suggested that the tumor has affected the expression of collagen and basement membrane of the cell.

Item Type: Article (Journal)
Additional Information: 5448/46358
Uncontrolled Keywords: Collagen, type IV collagen, melanoma cell lines, qPCR, gene expression
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Mardhiah Mohammad
Date Deposited: 23 Dec 2015 15:08
Last Modified: 01 May 2016 23:59
URI: http://irep.iium.edu.my/id/eprint/46358

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