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Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres

Doolaanea, Abd Almonem and Ismail, Ahmad Fahmi Harun and Mansor, Nur‘Izzati and Nor, Nurul Hafizah Mohd and Mohamed, Farahidah (2015) Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres. Tropical Journal of Pharmaceutical Research, 14 (10). pp. 1769-1778. ISSN 1596-5996 E-ISSN 1596-9827

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Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from polylactic glycolide (PLGA) microspheres. Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100, cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the impact of different surfactants on pDNA encapsulation. Results: Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fraction- dependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100 (HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100 exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate in DNA release. Conclusion: The microspheres were non-toxic to the neuro-2a cells which suggest they can be potentially used in the gene therapy of neuronal diseases.

Item Type: Article (Journal)
Additional Information: 7245/46259
Uncontrolled Keywords: Surfactant, Gene therapy, Microspheres, Polylactic glycolide, Plasmid DNA, Supercoil index, Hydrophilic-lipophilic balance
Subjects: R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Depositing User: Dr. Abd Almonem Doolaanea
Date Deposited: 16 Dec 2015 02:05
Last Modified: 16 May 2016 03:09
URI: http://irep.iium.edu.my/id/eprint/46259

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