Doolaanea, Abd Almonem and Ismail, Ahmad Fahmi Harun and Mansor, Nur‘Izzati and Nor, Nurul Hafizah Mohd and Mohamed, Farahidah
(2015)
Effect of surfactants on plasmid DNA stability and release from Poly (D, L-lactide-co-glycolide) microspheres.
Tropical Journal of Pharmaceutical Research, 14 (10).
pp. 1769-1778.
ISSN 1596-5996
E-ISSN 1596-9827
Abstract
Purpose: To evaluate the effect of surfactants on plasmid DNA during preparation and release from
polylactic glycolide (PLGA) microspheres.
Methods: Various surfactants, both ionic and non-ionic (Span, Tween, Triton X100,
cetyltrimethylammonium bromide and sodium dodecyl sulphate), were added during the microsphere
preparation and their effect was evaluated. Supercoil index (SCI) was introduced as a harmonised value
derived from encapsulation efficiency and supercoil preservation efficiency in order to evaluate the
impact of different surfactants on pDNA encapsulation.
Results: Polyvinyl alcohol and Span revealed low SCI whereas Tween increased the SCI in a fraction-
dependent manner. The Tween blend of hydrophilic-lipophilic balance (HLB) of 16 and Triton X-100
(HLB = 13.5) showed the highest SCI. Span revealed high burst release of pDNA whereas Triton X-100
exhibited low burst release. Following the burst release, diffusion mechanism was found to predominate
in DNA release.
Conclusion: The microspheres were non-toxic to the neuro-2a cells which suggest they can be
potentially used in the gene therapy of neuronal diseases.
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