An investigation into the antiobesity effects of morinda citrifolia l. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach

Jambocus, Najla Gooda Sahib and Saari, Nazamid and Ismail, Amin and Khatib, Alfi and Mahomoodally, Mohamad Fawzi and Abdul Hamid, Azizah (2016) An investigation into the antiobesity effects of morinda citrifolia l. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach. Journal of Diabetes Research, 2016. 2391592-1-2391592-15. ISSN 2314-6745

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Abstract

Theprevalence of obesity is increasingworldwide,with high fat diet (HFD) as one of themain contributing factors.Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism (alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment.

Item Type:	Article (Journal)
Additional Information:	6915/45851
Uncontrolled Keywords:	Insulin-resistance; lipoprotein-lipase; chinese medicine; systems biology; diabetic rats; weight-loss; nmr;serum; acid; metabolites
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	24 Nov 2015 14:06
Last Modified:	20 Oct 2017 22:56
URI:	http://irep.iium.edu.my/id/eprint/45851

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