Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae

Ahmad Yusof @ Hanafi, Hanani and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Osman, Malina and Jamal, Farida (2015) Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae. In: International Conference on Biomedical and Health Sciences Research 2015, 25-27 Jan 2015, The Everly Hotel Putrajaya. (Unpublished)

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Abstract

Streptococcus pneumoniae is an important pathogen cause various severe illness in human particularly pneumonia, septicaemia and meningitis. One of its virulence factors is hyaluronate lyase, an enzyme which degrades hyaluronic acid (HA), a major component in extracellular matrix of human connective tissues. Degradation of HA decrease host tissue viscosity and eventually increase tissue permeability to allow migration of pneumococcus or its product into human tissues. The study attempted to screen production of hyaluronate lyase in 100 isolates of S. pneumoniae from various sites and year of isolation, and other bacterial characteristics. Ninety-six isolates (96%) showed positive hyaluronate lyase through hyaluronic acid diffusion agar method. Few positive hyaluronate lyase isolates were selected and proceed to enzymatic activity determination through hyaluronic acid turbidity reduction assay. The study found that non-vaccine serotypes and carriage isolates showed high hyaluronate lyase activity than invasive isolates. This finding suggest that hyaluronate lyase in S. pneumoniae might play more important role in pneumococcal colonization than invasion.

Item Type:	Conference or Workshop Item (Poster)
Additional Information:	7232/45523
Uncontrolled Keywords:	Hyaluronate lyase, Streptococcus pneumoniae
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited:	03 Feb 2016 10:22
Last Modified:	03 Feb 2016 10:24
URI:	http://irep.iium.edu.my/id/eprint/45523

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