Genetic variation of wild tiger grouper (Epinephelus fuscoguttatus) obtained from Kedah, Malaysia

Waludi, Shaidatul Nadia and Phang, Ing Chia and Mukai, Yukinori (2015) Genetic variation of wild tiger grouper (Epinephelus fuscoguttatus) obtained from Kedah, Malaysia. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10th-12th August 2015, Impiana Resort Cherating, Pahang, Malaysia. (Unpublished)

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Abstract

The genetic variability among individuals of wild tiger grouper (Epinephelus fuscoguttatus) obtained from Kedah, Malaysia was examined by using random amplified polymorphic DNA (RAPD). Five samples of tiger grouper from FRI Pulau Sayak, Kedah, Malaysia were reared in Institute of Oceanography and Maritime Studies (INOCEM), Kampong Cherok Paloh, Pahang, Malaysia. The genomic DNA was extracted by using Qiagen Blood and Tissue kit. A total 155 of RAPD fragments was amplified. 87 polymorphic fragments (56.1%) with size ranging from 200 to 3000bp were scored. Low polymorphism percentage indicates of low genetic variation among the individuals of wild tiger grouper that caused by the small population size that could lead to loss of genetic biodiversity.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	6480/45471
Uncontrolled Keywords:	Epinephelus fuscoguttatus, genetic variation, polymorphism, RAPD, Phylogenetic tree
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Marine Science
Depositing User:	Dr. Yukinori Mukai
Date Deposited:	24 Nov 2015 14:33
Last Modified:	23 May 2018 08:31
URI:	http://irep.iium.edu.my/id/eprint/45471

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