A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics

Mohamad Puad, Noor Illi and Abd-Karim, Khairiah and Mavituna, Ferda (2015) A model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics. In: 28th Symposium of Malaysian Chemical Engineers (SOMChE 2015), 21st-22nd Oct. 2015, Palm Garden Hotel, IOI Resort Putrajaya. (Unpublished)

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Abstract

In this study, a kinetic model was developed for batch growth and sugar consumption by the cell suspension cultures of Arabidopsis thaliana (A. thaliana) Columbia (Col) ecotype cultivated in the continuous dark condition. The model included sucrose hydrolysis by the cell-wall invertase enzyme into glucose and fructose and consumption of these hexoses at different rates to support cell growth. Several assumptions were made in order to simplify modelling of a complex system. The whole living cells in the culture were treated as one uniform biomass by assuming that all individual living cells are of the same size, shape, mass, age and physiology. In addition, temperature and all concentrations in the system were considered as uniform. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5462/45320
Uncontrolled Keywords:	Arabidopsis thaliana, Columbia (Col) ecotype, kinetic model, cell growth, sugar uptake, Arabidopsis thaliana, ekotip Columbia (Col), model kinetik, pertumbuhan sel, penyerapan gula
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	02 Dec 2015 09:20
Last Modified:	28 Mar 2018 09:41
URI:	http://irep.iium.edu.my/id/eprint/45320

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