Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics

Mediani, Ahmed and Abas, Faridah and Khatib, Alfi and Tan, CP and Ismail, IS and Shaari, Khozirah and Ismail, Amin and Lajis, NH (2015) Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by H-1 NMR-based metabolomics. Industrial Crops and Products, 77. pp. 602-613. ISSN 0926-6690

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Abstract

tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus isa plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content.The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using proton nuclearmagnetic resonance (1H NMR) combined with multivariate data analysis (MVDA). The total phenoliccontent (TPC), DPPH radical scavenging activity and �-glucosidase inhibitory activity of the Phyllanthusspecies were also evaluated and correlated with their phytochemical constituents at different growthstages (8, 10 and 12 weeks) using partial least square regression (PLS). Principal component analysis(PCA) and PLS indicated separation between the two species based on the identified metabolites and thescreened bioactivities. A comparison of the two species indicated that P. urinaria was separated from P.niruri due to its larger quantity of fatty and amino acids, choline, phyllanthin and sucrose. However, P.niruri contained higher quantities of hypophyllanthin and phenolic compounds. The loading column plot,which was used to compare the P. niruri at different growth stages, indicated that the eight-week-oldplant contained a higher amount of fatty acids, amino acids (leucine and alanine), phyllanthin and choline.The dominant substances in the P. niruri at 10 weeks of growth by PC1 were identified as hypophyllanthin,malic acid, sucrose, and identified phenolics. The 12 week sample was differentiated by its higher sugarscontents as well as malic acid and leucine. The harvested samples of both Phyllanthus species at ten weeksof age exhibited significant bioactivities with the highest content and number of metabolites.

Item Type:	Article (Journal)
Additional Information:	6915/45216
Uncontrolled Keywords:	Phyllanthus species Metabolomics Growth stages Proton nuclear magnetic resonance Biological activities UPLC–MS/MS
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	20 Oct 2015 15:18
Last Modified:	16 Aug 2017 11:26
URI:	http://irep.iium.edu.my/id/eprint/45216

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