A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice

Zulaziz, N. and Azhim, Azran and Miyazaki, H. and Kinoshita, M. and Himeno, N. and Saitoh, D. and Morimoto, Y. (2015) A novel animal model for subcutaneous soft tissue infection using temporally neutropenic lys-EGFP mice. In: The 10th Asian Control Conference (ASCC 2015), 31 May-3 June 2015, Kota Kinabalu, Sabah.

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Abstract

Neutrophils play an important role against bacterial infection, mainly methicillin resistant Staphylococcus aureus (MRSA). Therefore, we developed an animal model to simultaneously monitor bacterial colonization and neutrophil migration in vivo. Using lys-EGFP C57BL/6 mice, we initially rendered the mice temporally neutropenic using cyclophosphamide (CPM) treatment (300mg/kg or 375mg/kg). Later, bioluminescent MRSA (Xen31, PerkinElmer) were subcutaneously injected (1.0×107 CFU) into the dorsal skin of the neutropenic lys-EGFP mice. The mice were then administered either saline (control group), or vancomycin (66mg/kg, treated group) consecutively for three days. For the evaluation of MRSA activity and neutrophil accumulation, an in vivo imaging system (LAS-4000, GE) was performed. Our results demonstrated that vancomycin is capable of killing bacterial cells and it also promotes inflammation. In addition, the rate of neutrophil regeneration after being suppressed by CPM is dependent on the dose of CPM. We could conclude that vancomycin is capable of inducing inflammation but the effects of the drug could only be observed if the immune system is adequately suppressed.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	7380/44920
Subjects:	T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Ir Dr Azran Azhim Noor Azmi
Date Deposited:	27 Jan 2016 02:29
Last Modified:	19 Jul 2016 11:13
URI:	http://irep.iium.edu.my/id/eprint/44920

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