Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures

Mohamad Puad, Noor Illi and Mavituna, Ferda (2015) Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures. In: The 22nd Regional Symposium on Chemical Engineering (RSCE 2015), 24th-25th Sept. 2015, Bangkok, Thailand.

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Abstract

A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism towards the formation of biomass. Assuming pseudo-steady state, mass balances were performed for each metabolite and the resulting equations were solved using linear optimization. The optimal metabolic flux distribution was determined in General Algebraic Modeling System (GAMS) 23.0 software by setting up the objective function as the maximization of biomass formation rate and using the constraints from batch experiments of A. thaliana suspended cell cultures. The results gave the theoretical maximum biological capacity of the cells to grow for a given specific uptake rate of sugar. The model proved to be a good starting point for more extended version of metabolism specifically for secondary metabolite production. Addition or extension of the model is feasible.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
Additional Information:	5462/44876
Uncontrolled Keywords:	Arabidopsis thaliana, Cell cultures, General Algebraic Modelling System (GAMS), Metabolic flux balance analysis, Primary metabolism
Subjects:	T Technology > T Technology (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User:	Noor Illi Mohamad Puad
Date Deposited:	23 Oct 2015 09:02
Last Modified:	24 May 2016 08:40
URI:	http://irep.iium.edu.my/id/eprint/44876

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