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Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

Farea, Manal and Husein, Adam and Halim, Ahmad Sukari and Berahim, Zurairah and Abdullah Nurul, Asma and Mokhtar@Makhtar, Khairani Idah and Mokhtar, Kasmawati (2015) Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells. Clinical Oral Investigation. ISSN 1436-3771 (O), 1432-6981 (P) (In Press)

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Abstract

Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.

Item Type: Article (Journal)
Additional Information: 7172/44845
Uncontrolled Keywords: Epithelial rests of Malassez cells . Cementogenic differentiation . Osteogenic differentiation . Stem cells of human exfoliated deciduous teeth . Transforming growth factor-β1
Subjects: R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Dentistry
Depositing User: Assoc Prof Khairani Idah Mokhtar
Date Deposited: 02 Oct 2015 12:23
Last Modified: 26 Feb 2016 09:06
URI: http://irep.iium.edu.my/id/eprint/44845

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