Farea, Manal and Husein, Adam and Halim, Ahmad Sukari and Berahim, Zurairah and Abdullah Nurul, Asma and Mokhtar@Makhtar, Khairani Idah and Mokhtar, Kasmawati (2015) Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells. Clinical Oral Investigation. ISSN 1436-3771 (O), 1432-6981 (P) (In Press)
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Abstract
Objectives The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β1 (TGF-β1) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED). Materials and methods SHED were co-cultured with ERM with/without TGF-β1. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated. Results TGF-β1 enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β1. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein). Conclusions ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β1. Clinical relevance The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.
Item Type: | Article (Journal) |
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Additional Information: | 7172/44845 |
Uncontrolled Keywords: | Epithelial rests of Malassez cells . Cementogenic differentiation . Osteogenic differentiation . Stem cells of human exfoliated deciduous teeth . Transforming growth factor-β1 |
Subjects: | R Medicine > RK Dentistry |
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Dentistry |
Depositing User: | Assoc Prof Khairani Idah Mokhtar |
Date Deposited: | 02 Oct 2015 12:23 |
Last Modified: | 26 Feb 2016 09:06 |
URI: | http://irep.iium.edu.my/id/eprint/44845 |
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