Identification of alpha-glucosidase inhibitors in Cosmos caudatus leaves using GC-MS based metabolomics

Khatib, Alfi and Javadi, Neda and Abas, Faridah and Abdul Hamid, Azizah and Simoh, Sanimah and Shaari, Khozirah and Ismail, Intan Safinar and Mediani, Ahmed (2015) Identification of alpha-glucosidase inhibitors in Cosmos caudatus leaves using GC-MS based metabolomics. In: 29th Scientific Meeting of Malaysian Society of Pharmacology and Physiolgy (MSPP), 24-25 August 2015, Setia City Convention Centre, Shah Alam, Malaysia. (Unpublished)

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Abstract

Cosmos caudatus (“Ulam Raja” in Malaysia) is a plant used in Malaysia to enhance vitality and has been studied for its antidiabetic activity. This study focused on the evaluation of the α-glucosidase inhibitory activity of different ethanolic extracts of C. caudatus. Six series of samples extracted with water, 20%, 40%, 60%, 80%, and 100% ethanol were employed. Gas chromatography-mass spectrometry (GC-MS) and multivariate data analysis was used to correlate bioactivity of different extracts to different metabolite profiles of C. caudatus. The obtained loading scores indicated a distinct and remarkable separation into 6 clusters, which were indicative of the 6 different quality of samples. GCMS can be integrated with multivariate data analysis to identify compounds that inhibit α-glucosidase activity. Through this approach several chemical makers e.g. catechin, α-linolenic acid, α-D-glucopyranoside, and vitamin E compounds were identified as an α-glucosidase inhibitors of this herb.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	6915/44594
Uncontrolled Keywords:	alpha-glucosidase inhibitors
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	09 Sep 2015 13:42
Last Modified:	10 Aug 2016 11:56
URI:	http://irep.iium.edu.my/id/eprint/44594

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